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Immunoblot analysis with vesicle-containing and particle-free culture supernatants. (A) Vesicle-containing culture supernatants of P. gingivalis strains were subjected to SDS-PAGE and immunoblot analysis with antigingipain, anti-Kgp, and anti-Hgp44 antibodies. (B and C) Particle-free culture supernatants of P. gingivalis strains were subjected to SDS-PAGE and immunoblot analysis with antigingipain antibody. WT, wild type; CBB, Coomassie brilliant blue.
