TABLE 1.
Identification of P. brasiliensis enolase by peptide mass fingerprinta
| Positionb | Identified amino acid sequencec | Mass |
|
|---|---|---|---|
| Exptl (in-gel digestion) | Theoretical (in silico digestion) | ||
| 16-32 | R.GNPTVEVDVVTETGLHR.A | 1,822.9489 | 1,822.9293 |
| 33-50 | R.AIVPSGASTGQHEACELR.D | 1,882.9548 | 1,825.8861 |
| 90-103 | K.VDEFLNKLDGTPNK.S | 1,589.8751 | 1,588.8678 |
| 106-120 | K.LGANAILGVSLAIAK.A | 1,410.9979 | 1,410.8678 |
| 164-184 | R.LAFQEFMIVPTAAPSFSEALR.Q | 2,325.2178 | 2,325.1947 |
| 243-254 | K.IALDIASSEFYK.A | 1,356.7701 | 1,356.7045 |
| 274-285 | K.WLTYEQLADLYK.K | 1,542.8513 | 1,542.7838 |
| 314-331 | K.TCDLQVVADDLTVTNPIR.I | 2,030.0239 | 1,973.0008 |
| 377-393 | R.SGETEDVTIADIVVGLR.A | 1,773.9489 | 1,773.9228 |
| 411-416 | K.LNQILR.I | 756.4464 | 756.4726 |
a
Protein scores higher than 76 are significant (P < 0.05). Peptide masses matched with PbEno (GenBank accession number EF558735.1), presenting a score of 113 and coverage of 34.25% of the whole deduced sequence.
b
Position corresponds to the peptide fragment detected by mass spectrometry.
c
The sequence within the periods represents the amino acids of the detected fragment. The amino acids outside of the periods represent the amino acids which remain within the protein following tryptic digestion.