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. 2010 Jul 12;78(9):3813–3821. doi: 10.1128/IAI.00247-10

FIG. 5.

FIG. 5.

Binding of NheC to Vero cells. Immunoblot of Vero cells exposed to NheC and the two mutated NheC proteins. Lane 1 shows an appropriate-size band of NheC consistent with NheC binding to Vero cells, whereas lanes 2 (NheCcys−) and 3 (NheChr−) do not. Lane 4 is the Vero cell preparation without added NheC, and lane 5 shows the NheC alone. Note that the lower-molecular-weight band in lanes 1 to 4 is a cross-reacting protein present in Vero cells.