FIG. 7.
Threshold inhibition of LDH release by NheC. (a) Concentration-dependent increase in LDH release from 106 Vero cells following exposure to increasing amounts of NheC added to 50 μl MHI1672 culture supernatants in 2-ml final volumes (mean ± standard error, n = 3 for each concentration). (b) LDH release from Vero cells after exposure to 5 ng (black bars) or 50 ng (gray bars) for 15 min before addition of AB (culture supernatant of MHI1672). The right-hand bars show LDH release when cells were washed before addition of AB, while the left-hand bars show LDH release when AB was added to the Vero cell monolayer without removal of excess C (mean ± standard error, n = 3 for each data set).