FIG. 4.

Mice protected from ECM still accumulate CD8⁺ T cells in the brain but not pRBC. P. berghei ANKA-infected mice were treated with pyrimethamine (Pyr) or vehicle (Vehic.) 20 h before the onset of CM. Brains were harvested after intracardiac perfusion at different days p.i., before (Bef) or after pyrimethamine treatment (A to C). Sequestration of CD8⁺ T cells was assessed either by qRT-PCR (A) or by flow cytometry (B) in independent experiments. (C) Brain sequestration of parasites was assessed by qRT-PCR. Data are representative of three independent experiments. Each symbol represents one individual mouse. n.s., not significantly different (Mann-Whitney test).