FIG. 5.

CD8⁺ T cells but not P. berghei NK65 parasites accumulate in the brain in a non-CM model. Mice were infected i.p. with P. berghei ANKA or P. berghei NK65. (A) Survival of P. berghei NK65-infected mice is shown. (B to D) At day 7 p.i., when P. berghei ANKA-infected mice developed CM, brains were harvested after intracardiac perfusion. In independent experiments, sequestration of CD8⁺ T cells was assessed by flow cytometry (B), quantitative brain capillary permeability was assessed in Evans blue-injected mice (C), and parasite mRNA expression was quantified by qRT-PCR (D). Data are representative of two independent experiments. Each symbol represents one individual mouse. n.s., not significantly different (Mann-Whitney test).