FIG. 1.
Genetic organization of the eight chromosomal loci acquired by Y. pestis and comparison with the homologous regions in Y. pseudotuberculosis. White arrows indicate CDSs present in Y. pestis CO92 but not in Y. pseudotuberculosis IP32953. Dark gray arrows indicate CDSs harboring >98% nucleotide identity between Y. pestis and Y. pseudotuberculosis. Light gray areas represent the conserved regions on each side of the acquired loci. Dashed lines with arrows below each region indicate the DNA fragments that have been replaced by a kanamycin cassette, and the numbers at each extremity correspond to the primer pairs used to generate the fragment to be deleted. Region not annotated are marked “Not ann.” In panel B, gray rectangles correspond to repeats identical in Y. pseudotuberculosis and Y. pestis. In panel F, the 250-nt-long DNA repeats found at the borders of R4pe are indicated by black rectangles. In panels E and H, black circles represent direct repeated sequences found at the extremities of each region, and their sizes in nucleotides are indicated below. In panel H, dashed arrows indicate ORFs that are conserved in Y. pestis and Y. pseudotuberculosis although they might have been acquired independently (see Fig. S1 in the supplemental material for a more detailed comparison of this region).