FIG. 4.

nHgbA_I/MPL antiserum immunoprecipitates only HgbA_I from whole cells of H. ducreyi. The ability of anti-nHgbA_I antisera to immunoprecipitate HgbA from different H. ducreyi strains is shown in a Coomassie blue-stained 10% SDS-PAGE gel (A) and a Western blot probed with an antiserum to full-length rHgbA_I (19) (B). The H. ducreyi strains used in these immunoprecipitation assays were 35000HPhgbA_I (1), 35000HPhgbA_II (2), and DMC111 (3). Antisera used in these experiments were either pooled anti-nHgbA_I/Freund's (Fp) purified from antisera obtained in a previous study (1) or pooled anti-nHgbA_I/MPL (Mp). Molecular size markers are shown in kilodaltons.