FIG. 6.

KIM5 Δy2313-y2316 and KIM5 ΔorfX mutants show decreased YopJ-dependent cytotoxicity. BMMs were infected with Y. pestis grown overnight at 28°C (A) or subcultured for 2 h at 37°C in the presence of 2.5 mM calcium (B) at an MOI of 5. BMMs were left uninfected (UI) or were infected with KIM5 or the indicated KIM5-derived strains (Table 1). Supernatants collected after 24 h of infection were used to measure cell death by LDH release assay. The data represent the averages of three independent experiments. Error bars represent standard errors of the mean (SEMs). The asterisks indicate a significant difference compared to the results for KIM5 Δy2313-y2316 (**, P < 0.01; ***, P < 0.001), as determined by one-way ANOVA with Tukey's multiple comparison test.