TABLE 1.
Yersinia strains used in this study
| Strain name | Relevant characteristicsa | Reference or source |
|---|---|---|
| KIM6+ | Pgm+ pCD1− pMT1+ pPCP1+ | 21 |
| KIM6+ Δy2313-y2316 | Deletion of y2313 nt 70-y2316 nt 903 | This work |
| KIM6+ ΔorfX | Deletion of nt 2550560-2550725 in Y. pestis KIM chromosomeb | This work |
| KIM6+ ΔfadAB | Deletion of fadA (y0463) nt 49-fadB (y0464) nt 1098 | This work |
| KIM6+ Δy655 | Deletion of y655 nt 37-504 | This work |
| KIM6+ Δy3553-y3555 | Deletion of y3553 nt 55-y3555 nt 368 | This work |
| KIM6 ΔpsaABC::aph pGFP | Δpgm ΔpsaABC::aph pGFP Kanr Apr | 35 |
| KIM6+ Δy2313-y2316/pComp | Δy2313-y2316 pMMB207 y2313-y2316 Camr | This work |
| KIM5 | Δpgm pMT1+ pPCP1+ pCD1Ap+ Apr | 33 |
| KIM5/pGFP | Δpgm pGFP Kanr Apr | 33 |
| KIM5 Δy2313-y2316 | Δy2313-y2316 Apr | This work |
| KIM5 ΔorfX | ΔorfX Apr | This work |
| KIM5 mJ | yopJ C172A (Cys172 to Ala172 codon change) Apr | 33 |
| KIM5 yopB | yopB18 (in-frame deletion of yopB nt 496-774) Apr | 33 |
| KIM5 Δy2313-y2316/pComp | Δy2313-y2316 pMMB207 y2313-y2316 Camr Apr | This work |
| KIM5 Δy2313-y2316/pVec | Δy2313-y2316 pMMB207 Camr Apr | This work |
| KIM5 mJ/yopJ-bla | yopJC172A pMMB207 yopJC172A-M45-bla Camr Apr | This work |
| KIM5 mJ Δy2313-y2316/yopJ-bla | Δy2313-y2316 yopJC172A pMMB207 yopJC172A-M45-bla Camr Apr | This work |
a
+, positive for indicated element; −, negative for the indicated element; nt, nucleotides.
b
The 165-bp deletion includes most of the putative orfX gene, starting immediately after the stop codon of the upstream flanking gene (y2316) and ending at nucleotide 120 of orfX.