TABLE 6.
Forty Y. pestis genes that are the most highly upregulated inside macrophages at 4 h postinfection
| Symbol | Locus taga | Predicted functionb | Fold change in expression level | Survival of deletion mutant in BMMsc |
|---|---|---|---|---|
| y1048 | 50S ribosomal protein L31 | 31.5 | ND | |
| fadB | y0464 | Four-enzyme protein involved in fatty acid oxidation | 29.4 | + |
| y2316 | Hypothetical protein | 26.6 | ++ | |
| mglB | y2662 | Periplasmic d-galactose-binding ABC transport protein | 24.1 | ND |
| ilvN | y2127 | Acetolactate synthase small subunit | 23.7 | ND |
| y2315 | Hypothetical protein | 21.0 | ++ | |
| ilvB | y2126 | Acetolactate synthase large subunit | 20.5 | ND |
| mglB | YPO1507 | Galactose-binding protein | 19.2 | ND |
| y1430 | Putative solute-binding periplasmic protein of ABC transporter | 19.1 | ND | |
| acs | y0510 | Acetyl coenzyme A synthetase | 18.8 | ND |
| y2313 | Hypothetical protein | 18.6 | ++ | |
| psaC | y2880 | Outer membrane usher protein | 18.0 | + |
| YPO3135 | 50S ribosomal protein L36 | 17.5 | ND | |
| argI | y0740 | Ornithine carbamoyltransferase | 17.4 | ND |
| ilvN | y2127 | Acetolactate synthase small subunit | 17.3 | ND |
| y1048 | 50S ribosomal protein L31 | 17.3 | ND | |
| fadE | y0946 | Acyl coenzyme A dehydrogenase | 17.2 | ND |
| cspD | YPO1366 | Cold shock-like protein | 17.1 | ND |
| hisJ | y1607 | Histidine-binding protein of histidine transport system | 16.6 | ND |
| y0655 | Hypothetical protein | 16.4 | + | |
| psaA | y2882 | pH 6 antigen fimbrial subunit | 15.4 | + |
| gltI | y1189 | Solute-binding protein of glutamate/aspartate ABC transporter | 14.8 | ND |
| glnK | y1041 | Nitrogen regulatory protein P-II 2 | 14.8 | ND |
| argC | y0310 | N-Acetyl-gamma-glutamyl-phosphate reductase | 14.0 | ND |
| hisJ | y1607 | Histidine-binding protein of histidine transport system | 13.6 | ND |
| rmf | YPO1423a | Putative ribosome modulation factor | 13.4 | ND |
| y3555 | Aspartate aminotransferase | 12.6 | + | |
| y0509 | Hypothetical protein | 12.3 | ND | |
| aceA | y0016 | Isocitrate lyase | 12.1 | ND |
| fadA | y0463 | Acetyl coenzyme A acetyltransferase | 12.1 | + |
| y0655 | Hypothetical protein | 11.8 | + | |
| mgtA | y1821 | Mg2+ transport ATPase | 11.8 | ND |
| psaB | y2881 | pH 6 antigen chaperone protein | 11.7 | + |
| ivbL | y2125 | ilvB operon leader peptide | 11.5 | ND |
| glnA | y3804 | Glutamine synthetase | 11.4 | ND |
| fadD | y2236 | Acyl coenzyme A synthase | 11.1 | ND |
| aceB | y0015 | Malate synthase | 10.7 | ND |
| YPO3681 | Putative insecticidal toxin | 10.6 | ND | |
| dctA | y3836 | C4-dicarboxylate transport protein | 10.5 | ND |
| y2591 | Hypothetical protein | 9.7 | ND |
a
Locus tags of Y. pestis genes from which the oligonucleotide probes are designed. Genes that appear twice on the list have two probes corresponding to them on the microarray. YPO designations are used for the probes that are based on CO92-specific sequences.
b
Predicted function is based on annotation by Pathogen Functional Genomics Resource Center/J. Craig Venter Institute.
c
+, bacterial replication in BMMs similar to that of wild-type bacteria; ++, increased intracellular replication; ND, not determined.