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. 2010 Jul 6;78(9):3898–3904. doi: 10.1128/IAI.00432-10

FIG. 3.

FIG. 3.

Regulation of dsrA promoter activity in the cpxA and cpxR deletion mutants during exponential growth. Whole-cell lysates of 35000HP(pKF2) (lanes 1, 4, and 7), 35000HPΔcpxA(pKF2) (lane 2, 5, and 8), and 35000HPΔcpxR(pKF2) (lanes 3, 6, and 9) were probed with an anti-GFP polyclonal serum and with the PAL-specific monoclonal antibody, 3B9. Cells were obtained after 1 h (lanes 1 to 3), 3 h (lanes 4 to 6), and 5 h (lanes 7 to 9) of growth. The solid arrow indicates GFP, and the open arrow indicates PAL. Normalized to PAL, the relative levels of expression of GFP for the cpxA mutant were 51, 63, and 82% that of the parent, respectively, after 1, 3, and 5 h of growth. The relative levels of expression of GFP for the cpxR mutant were 107, 126, and 154% that of the parent, respectively, after 1, 3, and 5 h of growth. The blot is representative of two independent experiments.