TABLE 2.
Oligonucleotides used in this study
| Primer | 5′ to 3′ sequencea |
|---|---|
| P1 | ATCCCTCGCAAGTTCGGGGGATTTTCTCTTTTTAAAGTATTTAATATATGATTCCGGGGATCCGTCGACC |
| P2 | ATAGACCGCTTTACCTAAAATGTATTAAATTATTCAAGCCAGAGTGGTAGTGTAGGCTGGAGCTGCTTCG |
| P3 | TATCTGCTCGGAGTTGCTTATCT |
| P4 | ACCGTTTCACCATTCTCGTT |
| P5 | AACGTTACCTTCAGCAAGCGGTTC |
| P6 | GCCGTTTGGGATCGTCGAGTGTATA |
| P7 | GCCATTCCTAATTTCGATGCGCGTTC |
| P8 | TTTGAGTGGCTACAGAAAGGCGAC |
| P9 | CCAAAGGTACCAAGCGTGTCTGTATCCCAC |
| P10 | GGGGATATCATTTAAATACAAGGTCAATGCTCGGC |
| P11 | GGAAGATCTCGCTACATCAGTTA |
| P12 | GGAAGATCTATTTGTTAAAGTGCTCAC |
| P13 | GGAAGATCTATTGGAGTGGACCAGGACAGCATT |
| P14 | GGAAGATCTAATCACCTCATTAAGTAAATAAT |
| P15 | CCTTCACCCTCTCCACTGACAG |
| P16 | TGGACTTGGTCTAATGAAGGCGGT |
| P17 | AAACGCCAGGAGCATATGTCACGA |
a
Boldfaced text represents sequences with homology to the mutagenic cassette. Underlining indicates regions corresponding to restriction enzyme sites as described in text. All H. ducreyi sequences are from GenBank accession no. AE017143.