FIG. 4.

Impact of Leishmania promastigotes from various species on STAT1α expression and activation. Adherent BMMs were infected with stationary-phase promastigotes of either L. donovani, L. major, or L. amazonensis for 6 h at a parasite-to-cell ratio of 20:1 and were then stimulated with 100 U/ml IFN-γ for the indicated times. Whole-cell lysates were separated by SDS-PAGE and analyzed by Western blotting using antibodies against pTyr701-STAT1 and the STAT1α C terminus, as described in Materials and Methods. Densitometric analysis was performed, and the results are expressed as the fold difference from the results for the unstimulated, uninfected sample. Data are representative of those from two independent experiments.