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. 2010 Jul 12;78(9):4012–4021. doi: 10.1128/IAI.00113-10

FIG. 2.

FIG. 2.

Effects of glucose/utilizable carbohydrates on ILY secretion by ΔccpA and ΔccpA/pccpA strains. (A) UNS38, ΔccpA, and ΔccpA/pccpA cells were inoculated onto human erythrocyte agar plates containing 0.1% or 1.0% glucose and then incubated at 37°C for 1 day. (B) Cells were grown for 24 h at 37°C in MOPS-BHI medium containing different concentrations of glucose (0.1 to 1.0%). (C) UNS38, ΔccpA, and ΔccpA/pccpA cells were grown for 18 h at 37°C in MOPS-BHI medium containing 0.1% glucose and 1.0% glycerol (Gly), fructose (Fru), maltose (Mal), or sucrose (Suc), and the OD600 of the cultures was measured. Standardized amounts of the culture supernatants were analyzed by 12.0% SDS-PAGE. Anti-ILY monoclonal antibody was used as a probe for immunodetection of ILY.