FIG. 6.

Effects of glucose/utilizable carbohydrates on ILY secretion by the cre mutant strains. (A) UNS38, 38 EM, creE, and creS cells were inoculated onto human erythrocyte agar plates containing 0.1% or 1.0% glucose and then incubated at 37°C for 1 day. (B) Cells were grown for 20 h at 37°C in MOPS-BHI medium containing different concentrations of glucose (0.1 to 2%). (C) UNS38, 38 EM, creE, and creS cells were grown for 15 h at 37°C in MOPS-BHI medium containing 0.1% glucose and 1.0% glycerol (Gly), fructose (Fru), maltose (Mal), or sucrose (Suc). Then, the OD₆₀₀ of the cultures was measured, and standardized amounts of the culture supernatants were analyzed by 12% SDS-PAGE. Anti-ILY monoclonal antibody was used as a probe for immunodetection of ILY.