TABLE 1.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| S. intermedius strains | ||
| UNS38 | ILY high-producing strain from human brain abscess | 36 |
| UNS38 B3 | ily knockout strain derived from UNS38 | 36 |
| UNS38 ΔccpA | ccpA knockout strain derived from strain UNS38 | Present study |
| UNS38 EMr | Insertion of EM cassette in front of ily promoter region of UNS38 | Present study |
| UNS38 creE | Same as UNS38 but with three point mutations in cre site | Present study |
| UNS38 creS | Same as UNS38 but with two nucleotide deletions and one point mutation in cre site | Present study |
| E. coli strains | ||
| C600 | thr-1 leuB6 thi-1 lacY supE44 rfbD1 fhuA21 | Lab collection |
| DH5αZ1 | F−endA1 hsdR17(rK− mK+) supE44 thi-1 recA1 gyrA(Nalr) relA1 Δ(lacZYA-argF)U169 deoR φ80dlacZΔM15 | 23 |
| Plasmids | ||
| pSET1 | S. suis replicating plasmid with Cm resistance | 37 |
| pSET1 Δlac | Deletion of lac promoter in pSET1 | Present study |
| pSET1 Δlac p15A | pSET1 Δlac carrying p15A replication origin | Present study |
| pccpA | pSET1 Δlac p15A carrying ccpA and putative native promoter | Present study |
| pUHE212-1 | IPTG-inducible expression vector with N-terminal six-His tag | 10 |
| pN-his ccpA | pUHE212-1 carrying ccpA | Present study |