TABLE 2.

Oligonucleotides used in the study

Target or purpose	Primer or probe	Sequence (5′-3′)
PCR primers
pSET1	pSET delta lac F	CTTGCATGCCTGCAGGTCGACTCTAGAG
	pSET delta lac R	CTCGCGCATGCGAAAGCCCCTAGAAGACG
p15A	p15A PstI	AGACTGCAGGGATATATTCCGCTTCCTCGC
	p15A SphI	ATCGCATGCGCTTGGACTCCTGTTGATAG
ccpA	ccpA EcoRI F	AAGAATTCAAAATGCTTGAAAGTGTTTCCAATAAGTG
	ccpA PstI R	GACTGCAGGCCTAACGGCCTCTTCTTTATTTCC
	his ccpA BamHI F	GAGGATCCATGAACACAGACGATACTG
	his ccpA PstI R	GCCCTGCAGTTATTTCCTTGTTGAACAACG
Disruption of ccpA	ccpA SalI F	CTATGGATGCGGTCGACTGTCTTGCAAAAGGCAACCG
	ccpA BamHI R	CTTTGGATCCAGCGTATTAACAACAGAGAC
	nested ccpA F	GTGATAAAATGATTTTGGTAGGAATGTGAAAACG
	nested ccpA R	GTTGAACAACGCTCAATCAGACCATGTGCC
Erythromycin cassette	erm (BamHI) F	AATGGATCCCCCGATAGCTTCCGCTATTG
	erm (SalI) R	CAGTAGTCGACCTAATAATTTATCTAC
Mutagenesis of cre	ily up F	AATACCAAGCCCAATGCAA CTCCTG
	ily up R	CTTTATAAGTCGACAGAAGCCCATTTTTCC
	ily promo. F	CTGTTTCTAACTAGATCTACTTCCCC
	ily no. 3 R	GTAGTCTGTGTTGTTTTGGATAGTTGC
	nested PCR F	CGACACAATGACTAAAGTGTATCACTCATC
	nested PCR R	GAGATTGGTACAGCTGGACTTTGAGCAC
	mt-cre EcoRI F	GAAAGAATTCGCAATTTAGCAAAAGGAGGC
	mt-cre EcoRI R	GCGAATTCTTTCATTTATATATTAACACTATGATGAGC
	mt-cre SphI F	GAAAGCATGCAATTTAGCAAAAGGAGGC
	mt-cre SphI R	ATTGCATGCTTTCATTTATATATTAACACTATGATGAGC
qRT-PCR for ily	qRT-ily F	CTATTAGTGTAAACTTACCGGGATTG
	qRT-ily R	GGACTATTTGGAGAGTCTACGCTAGC
qRT-PCR for gyrB	qRT-gyrB F	GATGAGGCACTAGCAGGTTTTGC
	qRT-gyrB R	GTGAACAGTTGTCCCTGTTCG
Probes for EMSA	cre sense	ATAAATGAAAGCGTTAGCAATTTAGCAAA
	cre antisense	TTTGCTAAATTGCTAACGCTTTCATTTAT
	cre EcoRI sense	ATAAATGAAAGAATTCGCAATTTAGCAAA
	cre EcoRI antisense	TTTGCTAAATTGCGAATTCTTTCATTTAT
	cre SphI sense	ATAAATGAAAGCATGCAATTTAGCAAA
	cre SphI antisense	TTTGCTAAATTGCATGCTTTCATTTAT
	non-cre sense	ATCGGTCTGTTATTTGTGTGTTTTTTATA
	non-cre antisense	TATAAAAAACACACAAATAACAGACCGAT