FIG. 2.

NF-κB activation in cells expressing TLRs by GXM. (A) Control systems. Pam₃CSK₄ (P₃C) and FSL-1, but not LPS, activated NF-κB nuclear translocation in cells expressing either TLR2/1 or TLR2/6, as expected. Transfection of HEK293A cells with a plasmid containing no TLR-coding sequences (vector) resulted in unresponsiveness. (B to F) Stimulation of HEK293A cells expressing TLR2/1 (inverted triangles) or TLR2/6 (triangles) by GXM samples resulted in dose-dependent NF-κB activation. Cryptococcus strains (serotypes) from which each GXM sample was isolated are given at the top of each panel.