FIG. 2.
Chitin utilization by Xylella fastidiosa. (A) Bacterial growth curve in XFM medium without carbon sources (XFM Δ) or with chitin added as the only carbon source (XFM Δ-chitin). Bars (second y axis) show the progression of chiA expression over time. (B) In-gel chitinase activity [4-MU(GlcNAc)3 cleavage] over time for X. fastidiosa culture filtrates in liquid XFM Δ-chitin, demonstrating ChiA secretion; (C) X. fastidiosa colonies grown on chitin-agar medium. The clear zones around colonies indicate chitin degradation. (D) In-gel chitinase activity (4-MU(GlcNAc)3 cleavage) for Escherichia coli strains grown in LB medium.