FIG. 2.

A. castellanii phagocytoses C. neoformans strains 24067, H99, and C536 and polystyrene beads. For imaging of extrusion, movies were recorded following incubation of amoeba and C. neoformans for 1 h and subsequent washing of extracellular C. neoformans. Recording then commenced, and the images represent still photos taken from movies at a magnification of ×10. (A) A. castellanii with ingested 5.2-mm polystyrene beads. (B) A. castellanii with ingested C. neoformans strain 24067. (C) A. castellanii with ingested C. neoformans strain H99. (D) A. castellanii with ingested C. neoformans strain C536. The large white arrow shows the internal replication.