FIG. 4.

Copper upregulation of czc2 during growth (A) and development (B). The strain was grown on CTT agar plates containing 0 μM (♦), 300 μM (▪), 600 μM (▴), or 800 μM (•) copper. The copper concentrations added to CF medium during development were 0 μM (♦), 20 μM (▪), 40 μM (▴), or 60 μM (•). The specific β-galactosidase activity in the cell extracts was determined as described in Materials and Methods. The results are the averages of data from three different experiments. The error bars indicate standard deviations. Note the differences in the scales.