Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PCR programs used in this study
| PCR | Initial denaturation temp (°C) (time [min]) | Denaturation temp (°C) (time [s]) | Annealing temp (°C) (time [s]) | Elongation temp (°C) (time [s]) | Final elongation temp (°C) (time [min]) |
|---|---|---|---|---|---|
| Legionella genus specific | 95 (15) | 95 (30) | 66.5 (30) | 72 (30) | 72 (10) |
| Legionella pneumophila | 95 (15) | 95 (45) | 60.0 (45) | 72 (45) | 72 (20) |
| Legionella 16S rRNA gene | 95 (15) | 95 (90) | 55.0 (40) | 72 (90) | 72 (10) |
