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. 2010 Jul 23;76(18):6257–6265. doi: 10.1128/AEM.00698-10

FIG. 2.

FIG. 2.

Metabolic flux study and cellulose chemical composition determination. (A) Quantification of cytosolic levels of UDP-glucose and UDP-GlcNAc in both engineered (+ operon) and control (- operon) G. xylinus cells fed either glucose or GlcNAc as the carbon source. Error bars represent standard deviations of three replicates (P < 0.0001). (B) Quantitative LC-MS/MS for measurement and the relative weight percent (wt%) of glucose and GlcNAc in acid-hydrolyzed BC and MBC extruded from either glucose- or GlcNAc-fed cultures. Error bars represent standard deviations of three replicates (*, P < 0.001). (C) Binding of fluorescent WGA to BC and MBC films.