TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | λ− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | 22 |
| OP50 | Nematode feeding strain | Caenorhabditis Genetics Center, University of Minnesota, MN |
| Y. enterocolitica | ||
| W22703 | Biovar 2, serovar O:9, Nalr Res− Mod+; lacking the pYV virulence plasmid | 10 |
| W22703 ΔtcaA | Mutant of W22703 with a non-polar deletion of tcaA | 7 |
| W22703 tcaA134::Tn5lux | Mutant of W22703 with tcaA knockout upon Tn5lux insertion | 7 |
| W22703 YE2848::Tn5lux | Mutant of W22703 with YE2848 knockout upon Tn5lux insertion | 7 |
| WA314/pYV | Biovar 1B, serovar O:8; Nalr; harbors virulence plasmid pYV | 40 |
| WA314/pYVΔyopE | Mutant lacking pYV-encoded effector protein YopE | 51 |
| WA314/pYVΔyadA | Mutant lacking pYV-encoded adhesin YadA | 38 |
| WA314 Δirp1 | Mutant lacking the chromosomally encoded yersiniabactin synthase HMWP1 | 36 |
| WA314 Δinv | Mutant lacking the chromosomally encoded invasin Inv | 40 |
| Y. pseudotuberculosis | ||
| STM 537 | Strain PB1, serovar O1a | 45 |
| STM 593 | Strain H-Y60/86, serovar O:2c | 3 |
| STM 542 | Strain YpIII(plB1), serovar O:3 | 54 |
| STM 595 | Strain H-Y52/86, serovar O:4 | 3 |
| S. enterica | ||
| Serovar Typhimurium | Wild-type strain ATCC 14028 | DSMZ, Braunschweig, Germany |
| Serovar Dublin | Wild-type strain 98-07710 | Robert Koch-Institut, Wernigerode, Germany |
| C. elegans | ||
| N2 (var. Bristol) | Wild-type strain | Caenorhabditis Genetics Center, University of Minnesota, MN |
| SS104 glp-4(bn2) | Temperature sensitive (sterile at 25°C) mutant | Caenorhabditis Genetics Center, University of Minnesota, MN |
| Plasmids | ||
| pNT-PYE2848::gfp | Promoter probe vector pNT with gfp fused to the low-temperature-induced promoter of YE2848; Kanr | 8 |
| pACYC184/F37.R43(s) | pACYC184 (9) with an EcoRI fragment containing tcaA and its promoter region; Tetr Cams | 7 |
| pBAD/HisA(tet) | pBAD/HisA (Invitrogen, Karlsruhe, Germany); Ampr exchanged for Tetr of pACYC184 | This study |
| pBAD/HisA(tet)-tcaA | pBAD/His(tet) harboring tcaA under the control of the arabinose promoter; Tetr | This study |
| pGreenTIR | Vector with gfp cloning cassette | 33 |