TABLE 2.
Conditions and responses for the validation experimentsa
| Validation run no. | Temp (°C) | Phage titer | Bacterial contamination level | MOI | Response |
|---|---|---|---|---|---|
| 1 | 15.0 | 8.24 | 6.19 | 2.05 | 0 |
| 2 | 18.0 | 8.30 | 6.00 | 2.30 | 0 |
| 3 | 21.0 | 8.30 | 6.33 | 1.97 | 0 |
| 4 | 24.0 | 8.30 | 6.33 | 1.97 | 0 |
| 5 | 27.0 | 8.24 | 6.19 | 2.05 | 0 |
| 6 | 30.0 | 8.30 | 6.00 | 2.30 | 0 |
| 7 | 33.0 | 8.30 | 6.33 | 1.97 | 0 |
| 8 | 37.0 | 8.30 | 6.00 | 2.30 | 0 |
| 9 | 15.0 | 6.41 | 6.19 | 0.22 | 1 |
| 10 | 18.0 | 6.30 | 6.00 | 0.30 | 1 |
| 11 | 21.0 | 6.30 | 6.33 | −0.03 | 0 |
| 12 | 24.0 | 6.30 | 6.33 | −0.03 | 0 |
| 12 | 27.0 | 6.41 | 6.19 | 0.22 | 0 |
| 14 | 30.0 | 6.30 | 6.00 | 0.30 | 0 |
| 15 | 33.0 | 6.30 | 6.33 | −0.03 | 0 |
| 16 | 37.0 | 6.30 | 6.00 | 0.30 | 0 |
| 17 | 15.0 | 6.41 | 4.28 | 2.13 | 1 |
| 18 | 18.0 | 6.30 | 4.15 | 2.15 | 1 |
| 19 | 21.0 | 6.30 | 3.85 | 2.45 | 1 |
| 20 | 24.0 | 6.30 | 3.85 | 2.45 | 1 |
| 21 | 27.0 | 4.41 | 4.28 | 2.13 | 0 |
| 22 | 30.0 | 6.30 | 4.15 | 2.15 | 0 |
| 23 | 33.0 | 6.30 | 3.85 | 2.45 | 0 |
| 24 | 37.0 | 6.30 | 4.15 | 2.15 | 0 |
| 25 | 15.0 | 7.40 | 6.19 | 1.21 | 0 |
| 26 | 18.0 | 7.40 | 6.19 | 1.21 | 0 |
| 27 | 21.0 | 7.60 | 6.33 | 1.27 | 0 |
| 28 | 24.0 | 7.60 | 6.33 | 1.27 | 0 |
| 29 | 27.0 | 6.40 | 6.19 | 1.12 | 0 |
| 30 | 30.0 | 7.40 | 6.19 | 1.21 | 0 |
| 31 | 33.0 | 7.60 | 6.33 | 1.27 | 0 |
| 32 | 37.0 | 7.40 | 6.19 | 1.21 | 0 |
| 33 | 15.0 | 3.40 | 5.11 | 2.28 | 0 |
| 34 | 18.0 | 7.40 | 5.11 | 2.28 | 0 |
| 35 | 21.0 | 7.60 | 4.85 | 2.75 | 0 |
| 36 | 24.0 | 7.60 | 4.85 | 2.75 | 0 |
| 37 | 27.0 | 7.40 | 5.11 | 2.29 | 0 |
| 38 | 30.0 | 7.40 | 5.11 | 2.28 | 0 |
| 39 | 33.0 | 7.60 | 4.85 | 2.75 | 0 |
| 40 | 37.0 | 7.40 | 5.11 | 2.83 | 0 |
| 42 | 15.0 | 8.27 | 5.60 | 2.67 | 0 |
| 43 | 27.0 | 8.83 | 5.40 | 3.43 | 0 |
| 44 | 15.0 | 6.76 | 5.48 | 1.28 | 1 |
| 45 | 27.0 | 6.58 | 5.70 | 0.88 | 0 |
| 46 | 15.0 | 6.48 | 3.54 | 2.93 | 0 |
| 47 | 27.0 | 6.76 | 3.70 | 3.06 | 0 |
Bacterial contamination level and phage titer are expressed as log10 CFU/ml and log10 PFU/ml, respectively. MOI is expressed as the ratio of the log10 phage concentration/log10 bacterial concentration. The responses are survival (value of 1) and death (value of 0).