TABLE 3.
Plasmids used in this studya
| Plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| pUC18 | AmpicillinR; E. coli subcloning vector | Invitrogen |
| pUC18-HXT1-del | pUC18 derivative carrying the PpHXT1 deletion cassette | This study |
| pPIC3.5K | AmpicillinR G418R; PAOX1-based expression vector | Invitrogen |
| pPICZ A | ZeocinR; PAOX1-based expression vector | Invitrogen |
| pRDM054 | AmpicillinR bleomycinR; vector carrying the PAOX1-BFP-SKL trifusion gene | 45 |
| pP-GFP | pPIC3.5K derivative carrying the GFP gene | 50 |
| pGS | pPIC3.5K derivative carrying the GFP-SKL fusion gene | This study |
| pBM2974 | AmpicillinR; URA3; PADH1-based expression vector | 30 |
| pBM3135 | pBM2974 derivative carrying the ScSNF3 gene | 30 |
| pBM3362 | pBM2974 derivative carrying the ScHXT1 gene | 30 |
| pZP10 | pBM2974 derivative carrying the PpHXT1 gene | This study |
| pZP20 | pBM2974 derivative carrying the PpHXT2 gene | This study |
a
R, resistance to indicated antimicrobial agent.