TABLE 1.
Strains, plasmids, transposons, and primers used in this study
| Strain, plasmid, or primer | Description and/or relevant genotype or primera | Reference or source |
|---|---|---|
| Strains | ||
| M. mediterranea | ||
| MMB-1R | Wild type; spontaneously Rifr | 39 |
| T103 | MMB-1R ppoS::Tn10 Kmr | 26 |
| MMB1LAC0 | MMB-1R Ω mini-Tn10 Gmr (trp′-′lacZ) | 26 |
| T103LAC0 | T103 Ω mini-Tn10 Gmr (trp′-′lacZ) | 26 |
| MMB1LACL1 | MMB-1R Ω mini-Tn10 Gmr φ[PlodAB(252 bp)-lacZ] | This study |
| MMB1LACL2 | MMB-1R, Ω mini-Tn10 Gmr φ[PlodAB(115 bp)-lacZ] | This study |
| T103LACL1 | T103 Ω mini-Tn10 Gmr φ[PlodAB(252 bp)-lacZ] | This study |
| LD (lodAB mutant) | MMB-1R ΔlodAB | P. Lucas-Elío et al., unpublished results |
| LDAB | LD Ω mini-Tn10 Gmr (lodAB) | 16 |
| LDAHa/B-1 | LDB Ω mini-Tn10 Kmr (lodA-Ha-Ha-His6) | 16 |
| E. coli S17-1(λpir) | Kmr::Tn7 Tpr SmrrecA ths hsdRM+; λpir phage lysogen RP4::Mu::Km Tn7 | 8 |
| Plasmids | ||
| pBFU8 | pBSL 182 mini-Tn10 Gmr φ(PppoA-lacZ); delivery vector | 26 |
| pBPlod1 | pBSL182 mini-Tn10 Gmr φ[PlodAB(252 bp)-lacZ]; delivery vector | This study |
| pBPlod2 | pBSL182 mini-Tn10 Gmr φ[PlodAB(115 bp)-lacZ]; delivery vector | This study |
| Primers | ||
| MAREV3 | 5′-GCCCATAAAGAAGGTTGCC-3′ | |
| AAP | 5′-GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG-3′ | Invitrogen |
| MAREV4 | 5′-CACCCAATACAATAAGGCG-3′ | |
| AUAP | 5′-GGCCACGCGTCGACTAGTAC-3′ | Invitrogen |
| MAREVsal | 5′-CACTTGTCCCTGTCGACG-3′ | |
| MARDIReco3 | 5′-GGTTGAATTCTGTTATTGAGACGGTC-3′ | |
| MARDIReco4 | 5′-CGTTGGGGAATTCAGAGAAACAGCCTC-3′ | |
| MAREV3bam | 5′-TGTTGATAACTAGGATCCATAAACCGTT-3′ | |
| GmREV2 | 5′-GGACCAGTTGCGTGAGCG-3′ | |
| LacZREV | 5′-GGTTTTCCCAGTCACGACG-3′ |
a
For the sequence of primer AAP, “I” refers to deoxyinosine. Engineered restriction sites are underlined.