TABLE 2.
List of loci and primers used for PCR amplification
| Locus | Expected product size (bp)a | Hot startb | Primer name and sequence (5′-3′) |
|
|---|---|---|---|---|
| Forward | Reverse | |||
| ldh | 921 | No | HdB35ldhF, CAAAAAATTATTTTAGTTGGCGACGGAGCAGTTGG | HdB36ldhR, TTGTTTCATTGCRTCGTCWAG |
| lmo0490 | 791 | Yes | HdB37LMO490F, GCCACTCCAATCAGACACAGTTTATCACCAAC | HdB38LMO490R, ACTGGCATTTCTTTGTGTGTCCAAATTTCGAAAGC |
| prs | 809 | No | HdB40prsF, CCAAATTAACATTGAAGAAAGTATCCGTGGTTGTC | HdB41prsR, GAACTTACAGAWGCATTYTCATGWAC |
| sigB | 841 | Yes | Lm sigB15, AATATATTAATGAAAAGCAGGTGGAG | Lm sigB16, ATAAATTATTTGATTCAACTGCCTT |
| polC | 968 | No | HdB14polCF, CAAGCWGCTGATTGGGGWCAYAAAGC | HdB15polCR, GCMGCTTGCATYCCTTTTTGCATCATYGCTTCA |
| rarA | 860 | No | HdB30rarAF, ATGGCAATTCAACCTTTAGCTTACCGRATGCG | HdB31rarAR, TCCTCATAWGCCATAACDAGCATTCTCC |
| LMO1555 | 712 | No | HdB32LMO1555F, ATGAKTAAAAAARTTRTTTTAACAAGAGARGC | HdB33LMO1555R, CCTCCTGTATTATCAAATCAGCTARGTGCTTCATG |
| pbpA | 1,240 | Yes | HdB20pbpAF, TCGCAGAAGTTGGAACCGAACGGCGGG | HdB21pbpAR, GCAGCATARGCWCCRGCCATTTGCATTGG |
| addB | 845 | No | HdB26addBF, TGGAAATGGGAAAAAGARGGMGAYTGGAT | HdB27addBR, GCTTGYTTAGARCGAATRTAACTACTTTGTTC |
| LMO2763 | 1,216 | Yes | HdB39LMO2763F: GAACACGGCATGGAACGTGTTTTAGTACCAG | HdB40LMO2763R, AGCCGACCAGAAGCGCGAGCCAGTTTCCTCCTG |
a
The expected product size was calculated based on the respective gene sequence for L. monocytogenes EGD-e.
b
This column indicates whether hot start PCR was performed (hot start PCR was done by manual addition of the Taq polymerase after the initial denaturation step).