TABLE 1.

Estimated genotypes, coverage percentages, and diversity indices of narG and nosZ amino acid sequences from the alimentary canals of earthworms and from soils^a

Gene	Library	No. of sequences	No. of OTUs	Coverage (%)	Diversity indices
					Richness	H′b	Evenness	1/Dc
narGd	DNA, guts	57	5	100	5 ± 0	1.30	0.81	2.95
	DNA, soils	82	5	100	5 ± 0	1.16	0.72	2.33
	cDNA, guts	82	3	100	3 ± 0	0.55	0.50	1.45
	cDNA, soils	26	3	100	3 ± 0	1.06	0.97	3.00
	DNA, total	139	8	99.3	8 ± 0	1.41	0.68	2.67
	cDNA, total	108	4	100	4 ± 0	0.82	0.59	1.80
	All narG sequences	247	9	99.6	10 ± 0	1.57	0.71	3.75
nosZe	DNA, guts	70	11	98.6	12 ± 1	1.53	0.64	2.75
	DNA, soils	19	8	84.2	10 ± 1	1.84	0.88	6.33
	cDNA, guts	39	9	92.3	11 ± 1	1.74	0.79	4.72
	cDNA, soils	29	13	72.4	20 ± 3	2.08	0.81	5.64
	DNA, total	89	15	95.5	17 ± 1	1.78	0.66	3.23
	cDNA, total	68	18	83.8	41 ± 13	2.15	0.74	5.68
	All nosZ sequences	157	26	92.4	66 ± 48	2.15	0.66	4.30

^aAll calculations were carried out with DOTUR 1.53 and are based on amino acid sequences as described in Materials and Methods.

^bH′, Shannon-Weaver diversity index.

^c1/D, reciprocal Simpson's diversity index.

^dnarG at a species level cutoff value of 41% dissimilarity of amino acid sequences.

^enosZ at a species level cutoff value of 14% dissimilarity of amino acid sequences.