FIG. 7.

Charge reversal mutations in cytochrome c retain residual suppressor activity for coa4Δ cells but not cyc1Δ cyc7Δ mutant cells. (A) Respiratory growth of the BY4743 wild-type and coa4Δ cells transformed with episomal vectors expressing wild-type and mutant forms of CYC1 or wild-type CYC7 was analyzed as described in the legend to Fig. 1. (B) Respiratory growth of the BY4743 wild-type and shy1Δ cells transformed with episomal vectors expressing wild-type CYC1 and CYC7. (C) Expression of the wild-type and mutant forms of Cyc1 in whole-cell extracts assessed by immunoblotting. Whole-cell proteins were precipitated with 20% trichloroacetic acid (TCA) and resolved on 15% SDS-PAGE. Pgk1 was used as a loading control.