FIG. 4.

Effects of convergent transcription and length of CAG repeats on cell death. (A) Convergent transcription and cell death in various cell lines. Cell lines were treated for 4 days with or without Dox plus RSL1. (B) Correlation between CAG tract length and cell death induced by convergent transcription; (C) convergent transcription and apoptosis in DITS-H cells. DITS-H5 and DITS-H8 cells were treated with DMSO, doxycycline plus DMSO, or doxycycline plus zVAD for 7 days. Percentages of viable cells were calculated as described in the legend to Fig. 3 and are averaged from at least six independent measurements. Error bars show standard deviations. Statistical significance is indicated: ***, P < 0.0001.