TABLE 1.
Sequences of real-time RT-PCR primers and siRNAs used in this study
| Gene product | Real-time RT-PCR primer sequence |
siRNA sequence | |
|---|---|---|---|
| Forward | Reverse | ||
| β-Actin | AGAGAGGCATCCTCACCCTG | CATGAGGTAGTCAGTCAGGT | |
| ATM | GCAGCATGGAGGAATATGCAG | CTGCGCTTACACATCTCTTCC | GCAACAUUUGCCUAUAUCAGCAAUU |
| CACAAGGTCAGGAGTTCGAG | TCACCTCCCAGGTTCAAGAG | GCGCAGUGUAGCUACUUCUUCUAUU | |
| ATR | TGCTGTTGTCTTACAACTGACTG | CAGTGCCAGTTTCTGAACAG | CCACCUGAGGGUAAGAACAUGUUAA |
| CAGGGCTATTCAGCTAGAACC | CTGCTTCCACTCTGTACGTG | GGGAAAUACUAGAACCUCAUCUAAA | |
| ATRIP | CATCAGACGGAATCCGTTCTAG | CTTTGTCCTTAATTCATTCATCTCTGC | UGACCAGCAGGAACUGCCCAGAGAA |
| GGTTCACAGGCTTAGTGATGG | ACTGGCTTGAAGGTGACC | ||
| CHK1 | AGCCAGACATAGGCATGC | CGAAATACTGTTGCCAAGCC | CCCAGCCCACAUGUCCUGAUCAUAU |
| GTACTCCAGTTCTCAGCCAG | GGTTCTGTGAGGATCCTGG | GGCUUGGCAACAGUAUUUCGGUAUA | |
| CHK2 | CTCCTCTACCAGCACGATG | TCCATCCTGAAGGGCC | GAACCUGAGGAGCCUACCCUU |
| AAGAACCUGAGGACCAAGAACUU | |||
| p53 | AGATGAAGCTCCCAGAATGC | AAACCGTAGCTGCCCTG | GCUUCGAGAUGUUCCGAGAGCUGAA |
| GGAGGCCCATCCTCAC | GCGGAGATTCTCTTCCTCTG | CCGGACGAUAUUGAACAAUGGUUCA | |
| TOPBP1 | GGCTGTTGGAGACTGCTAG | CGTGTGCCAGGATGCTC | GCCUUUACAUGAUUCAGAAAUUGCU |
| AGGCAGTCTCGTCAGACAG | GCCACTGCAAATTGCTGG | ||
| Vimentin | GAAUGGUACAAAUCCAAGU | ||