TABLE 3.
Relative sense and antisense transcript levels of HPRT minigene
| Cell line | Relative transcript level in cellsa: |
|||
|---|---|---|---|---|
| Untreated |
Treated with doxycycline + RSL1 |
|||
| Sense | Antisense | Sense | Antisense | |
| DIT7 | 1.0 ± 0.1 | 1.0 ± 0.2 | 22 ± 3.1 | 16 ± 3.3 |
| DIT3 | 0.7 ± 0.1 | 2.2 ± 0.3 | 15 ± 0.5 | 9.7 ± 3.0 |
| FLAH9 | 0.4 ± 0.3 | 16 ± 4.2 | ||
| FLAH25 | 0.6 ± 0.2 | 14 ± 2.7 | ||
| DIT-NAT10 | 0.3 ± 0.1 | 0.2 ± 0.1 | 19 ± 3.6 | 0.5 ± 0.2 |
| DIT-NAT26 | 0.8 ± 0.2 | 0.2 ± 0.1 | 25 ± 5.3 | 0.3 ± 0.1 |
| DIT-NR4 | 0.2 ± 0.1 | 1.2 ± 0.3 | 10 ± 0.5 | 11 ± 0.7 |
| DIT-NR5 | 0.6 ± 0.1 | 1.3 ± 0.5 | 19 ± 2.3 | 14 ± 3.2 |
| DH7 | 1.1 ± 0.1 | 38 ± 8.3 | 11 ± 1.1 | 36 ± 7.7 |
| DH10 | 0.2 ± 0.1 | 24 ± 4.0 | 12 ± 2.3 | 26 ± 1.2 |
| DIT7-R15 | 25 ± 5.9 | 14 ± 2.5 | ||
| DIT7-R20 | 24 ± 4.2 | 16 ± 4.2 | ||
| DIT7-R25 | 27 ± 4.8 | 17 ± 2.8 | ||
| DIT7 (starved) | 2.5 ± 0.4 | 1.9 ± 0.5 | 17 ± 1.3 | 7.2 ± 0.5 |
| DIT3 (starved) | 2.8 ± 0.3 | 1.8 ± 0.2 | 12 ± 1.4 | 9.3 ± 0.9 |
| DIT7 (confluent) | 1.6 ± 0.4 | 1.3 ± 0.3 | 24 ± 4.7 | 20 ± 3.5 |
| DIT7 (zVAD) | 21 ± 3.8 | 16 ± 2.9 | ||
a
The levels of sense and antisense transcription in DIT7 cells before induction were arbitrarily defined as 1 and used as the basis for comparison. Shown here are the relative levels of sense and antisense transcripts in various cell lines, which were determined using strand-specific RT-PCR as described in the legend to Fig. 1H.