FIG. 1.
Asp1 kinase activity increases resistance towards TBZ. (A) Diagrammatic representation of wild-type Asp1 (top) and the Asp1 variant that rescues the mal3 mutant TBZ phenotype. Note that the conserved histidine residue H807 is not present in Asp11-794. (B) Serial dilution patch test of a mal3 mutant strain expressing asp11-794 or mal3+. Cells were spotted on plasmid-selective plates containing 8 μg/ml TBZ and incubated for 7 days at 24°C. (C) Wild-type-, asp1D333A-, or asp1H397A-expressing cells were grown on YE5S containing 9 μg/ml TBZ for 5 days at 24°C.