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Calcineurin regulation of PDE4D degradation is phosphodegron dependent. Wild-type or phosphodegron-mutated (Ala616, Ala618, Ala616,618, or Ala621,622,623) PDE4D3 was coexpressed with CnAβ in COS7 cells. The expression levels of PDE4D3 and CnAβ were determined by immunoblot analysis. The expression level of tubulin was used as a control.
