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. 2010 Jul 6;30(17):4245–4253. doi: 10.1128/MCB.00218-10

FIG. 6.

FIG. 6.

Early embryonic lethality caused by deficiency in N6amt1. (A) H&E staining of longitudinal sections of E6.5 embryos from intercrosses of N6amt1+/− mice. Typical embryos with normal (left) and abnormal (right) morphologies are shown. The normal E6.5 embryo has initiated the formation of a primitive streak (ps) and developed an elongated egg cylinder. The abnormal embryo is much smaller in overall size. The ectoplacental cone (epc) is underdeveloped, and no primitive streak forms. Endoderm and ectoderm cannot be distinguished. xen, extraembryonic endoderm; xec, extraembryonic ectoderm; een, embryonic endoderm; eec, embryonic ectoderm. Scale bar, 50 μm. (B) Outgrowth of blastocysts from N6amt1+/− intercrosses in vitro. E3.5 blastocysts were collected and cultured in ES medium for 9 days. Genotypes were determined by genomic PCR. ICM and trophoblastic giant cells (TGC) in outgrowth at day 9 are pointed out. Scale bars, 100 μm.