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. 2010 Jun 21;30(17):4149–4158. doi: 10.1128/MCB.00224-10

FIG. 5.

FIG. 5.

The growth-inhibitory effects of Aiolos and Ikaros on pre-B cells are compromised in the absence of p27. p27+/+ and p27−/− pre-B cells were infected with Aiolos and Ikaros. (A) The effect of Aiolos and Ikaros on cell cycle progression was analyzed 48 h later by FACS. Infected cells expressing a high level of GFP (GFP+) and the GFP-negative (GFP−) cells were analyzed separately. (B) The expression of λ5 was determined in GFP+ and GFP− cells by real-time PCR analysis. (C) Bone marrow cells from p27+/+ and p27−/− mice were stained with antibodies to B220, CD25, and IgM. After surface staining, the cells were incubated with Hoechst dye 33342 and analyzed by FACS. The cell cycle status of the pre-B cells (B220+ CD25+ IgM−) was examined. In addition, pre-B cells were further fractionated into small pre-B and large pre-B categories based on the forward scatter. The numbers indicate the percentages of large pre-B cells in p27+/+ and p27−/− mice. The result is representative of at least three independent experiments. The statistical significance was determined by a Student t test (*, P < 0.05; **, P < 0.01).