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. Author manuscript; available in PMC: 2010 Sep 13.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2004 Mar;45(3):764–768. doi: 10.1167/iovs.03-1114

Figure 2.

Figure 2

Immunoblot analysis of various photoreceptor markers in normal retinas, PE, and 661W cells. Twenty-microgram aliquots from 661W cells and C57BL/6 retinal or RPE lysates were separated on 12% polyacrylamide gels and blotted onto PVDF membranes and immunoreacted with anti-rod opsin (Rho, A), rod arrestin (rArr, B), phosducin (Phos, C), RPE65 (D), peripherin/rds (P/rds, E), ROM1 (F), and rod transducin (GαT1, G). The results show a positive immunoreaction for all antigens in normal retinal extracts (and PE in RPE65) but an absence of reactivity in extracts from 661W cells.