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. Author manuscript; available in PMC: 2010 Sep 13.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2004 Aug;45(8):2514–2521. doi: 10.1167/iovs.04-0065

FIGURE 4.

FIGURE 4

Critical levels of P/rds needed for maintaining photoreceptor function and structure. (A) Blot analysis demonstrated the increase in P/rds protein expression in NMP+/−/rds+/− relative to rds+/− and reflected a level corresponding to 80% of wild type. (B) Scotopic and (C) photopic ERG analyses at 7 months of age showed a significant improvement in rod and cone ERG amplitudes, respectively, in NMP+/−/rds+/− when compared with rds+/− mice. ERG results are an average of 12 to 16 eyes for each genotype. (D) Retinal histology and EM of 7-month-old mice revealed a considerable and long-lasting amelioration in OS structure in NMP+/−/rds+/− relative to rds+/− mice. Light microscopy histology (top) shows an outer nuclear layer count of 9 to 10, 6 to 7, and 5 to 6 rows in wild type, NMP+/−/rds+/−, and rds+/− retinas, respectively. Bottom: electron micrographs. Scale bar: (D, top) 20 µm; (D, bottom) 4 µm.