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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Exp Eye Res. 2010 May 4;91(2):186–194. doi: 10.1016/j.exer.2010.04.017

Figure 5. Nrl and Nr2e3 regulation of Rds expression.

Figure 5

a. Y-79 cells were co-transfected with the luciferase construct (and pCHIP110) and, as indicated, pNrl. Expression of Nrl led to a significant increase in Rds gene expression. b. Rds mRNA levels (qRT-PCR) were significantly reduced in Nr2e3-/- mice and significantly increased in Crx-Nrl mice. * p<0.01. c. Western blots on 5 μg of total retinal extract from WT or Crx-Nrl mice were probed with polyclonal antibodies against the RDS C-terminal (RDS-CT) and with antibodies against actin. RDS protein levels (when normalized to actin) were increased by approximately 2-fold.