TABLE 1.

Primer sequences used in construction of the expression vectors

Primer name	Size (bp)	Tma(°C)	Sequence
s309for	31	77.6	5′ GCG CAT GCA TCC ATG TTT AGG AGC CCG GAG G 3′
s309rev	29	73.2	5′ GCG CCT CGA GTT AAA CAA GCA TCA GCA CG 3′
T309for	30	72.1	5′ AAA AGG CGC GCC AAG CTT GGT ACC ATG TTT 3′
T309rev	29	71.8	5′ GGG AAA AAG CC ACCT TCC TCT GCA GCA TT 3′
H262Sfor1	25	69.5	5′ GGG GGG TAC CAT GTT TAG GAG CCC G 3′
H262Srev1	29	68.1	5′ CCC CAT CGA TAC CGT CTC CTT CTC GAA AA 3′
H262Sfor2	26	71.1	5′ GGG GAT CGA TCC CGT CCC AGT GCA TC 3′
H262Srev2	27	68.0	5′ GGG GGC ATC AGG CGA AAT TGT AAA CGG 3′
D303for1	29	72.3	5′ GGG GAA AAC ACA CGT GCC TGG ACC TCT CC 3′
D303rev1	30	73.6	5′ GGG GAT GCA TCA GCT CGA CTC TAG CCG CAG 3′
D321rev1	30	72.2	5′ GGG GAT GCA TCA GAT CTC GAG CTC GTC CAC 3′
D327rev1	30	72.2	5′ GGG GAT GCA TCA CTC GTT CGT CTC GTT GGG 3′
Sak1for	30	75.0	5′ GGG GAC CGG TCG AGA AGA GGA GGA AGG AGG 3′
Sak1rev	30	70.9	5′ GGG GAC CGG TGC TCC TAA ACT TAT CGT CGT 3′
SakFlagfor	39	79.9	5′ GGG GAC CGG TCG AGA AGA TGC TCC TAA ACT TAT CGT CGT 3′
SVTinsfor	38	84.0	5′ GGG GGG GGC GCG CCA AGC TTG GTA CCA TGT TTA GGA GC 3′
CMVSak	19	67.4	5′ GCC CAA CGA CCC CCG CCC A 3′
M53for1	19	61.0	5′ CAC ACG TGC CTG GAC CTC T 3′
M53rev1	19	61.0	5′ AGA GGT CCA GGC ACG TGT G 3′
Pack1for	20	67.0	5′ TGC ATC GAC GGT CCC AGC CA 3′
Pack1rev	20	65.0	5′ CCG CGG TGG TCC CCA TTG TG 3′

^aT_m, melting temperature.