FIG. 3.

Interaction of TYLCV CP with B. tabaci GroEL proteins in yeast. (Top) Selection for growth in the presence of leucine as a control for the quality of the cells. The growth of yeast cells cotransfected with TYLCV CP-containing plasmid and the various GroEL plasmids (Hamiltonella, Portiera, Rickettsia, the GroEL gene reported by Morin et al. [31] as a positive control and the pLexA-BD + pB42-AD plasmids without any genes as a negative control) are shown. (Bottom) Growth of the same cotransfected yeast cell lines with medium lacking Leu. Growth or the lack of growth of three independent yeast colonies is shown for each cotransfection.