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. 2010 Jul 14;84(18):9310–9317. doi: 10.1128/JVI.00423-10

FIG. 6.

FIG. 6.

Recombinant Hamiltonella-GroEL interaction with TYLCV CP in whole whiteflies and hemolymph extracts but not in salivary glands. (A) Whole whiteflies (females). Lanes 1 and 2 show the results of IC-PCR with GroEL antibody and extracts from whole B-biotype viruliferous females from two strains (B-HRc and B-HRs, respectively), and lanes 3 and 4 are no-antibody controls. Lanes 5 and 7 show the results of IC-PCR analysis with GroEL antibody and extracts from two Q-biotype strains (Q-WA and Q-RA AV04E, respectively) that were fed for 24 h on in vitro-synthesized Hamiltonella GroEL, and lanes 6 and 8 are no-antibody controls. Lane 9 contains a positive PCR control plasmid (bearing the full TYLCV sequence), and lane 10 contains a positive PCR control from a TYLCV-infected plant. Lane 11 is a negative no-template control. Lane M contains molecular size markers. (B) Hemolymph. Lanes 1 and 2 show the results of IC-PCR with GroEL antibody and extracts of hemolymph from strains B-HRc and B-HRs, respectively, and lanes 3 and 4 are no-antibody controls. Lanes 5 and 7 show the results of IC-PCR analysis with GroEL antibody and hemolymph extracts from strains Q-WA and Q-RA AV04E, respectively, fed on recombinant Hamiltonella GroEL for 24 h, and lanes 6 and 8 are no-antibody controls. Lane 9 is a negative no-template control. (C) Salivary glands. Lanes 1 and 2 show the results of IC-PCR with GroEL antibody and extracts from salivary glands of viruliferous B-biotype (B-HRc and B-HRs, respectively) females. Lanes 3 and 4 show the results of IC-PCR with TYLCV CP antibody and extracts from salivary glands of the same viruliferous B-biotype females. Lanes 5 and 6 show the results of IC-PCR with GroEL antibody and extracts from salivary glands of viruliferous Q-biotype (Q-WA and Q-RA AV04E) females. Lanes 7 and 8 show the results of IC-PCR with TYLCV CP antibody and salivary glands extracts from the same viruliferous Q-biotype females. Lane 9 is a negative control (TYLCV-free plant). Lane 10 is a positive control (TYLCV-infected plant). Lane 11 contains plasmid (bearing the full TYLCV sequence) and is a positive control.