FIG. 2.

Binding of P20 to the HIV-1 gp41 6-HB core. (A) Binding of P20 to the N peptide N36, the C peptide C34, and the 6-HB 3NC, as determined by direct ELISA. (B) Binding of P20 to 6-HB, as detected by sandwich ELISA using the 6-HB-specific MAb NC-1. Gelatin was used as the negative control. (C) Kinetic analyses of P20 and 6-HB interaction by SPR. The y axis of the sensorgram is denoted as the RU signal, whereas the time (in seconds) is represented on the x axis. A series of concentrations of P20 were injected at a flow rate of 5 μl/min. Experimental curves (solid line) are shown overlaid with fitted curves (dash line) obtained with the 1:1 binding-with-drifting baseline model.