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Effect of P20 on the gp41 6-HB formation between N36 and C34 by ELISA (A) and FN-PAGE (B). ADS-J1 was used as a positive control. (a) After native polyacrylamide gel electrophoresis, the gel was visualized using a transillumination UV light source to reveal the bands containing C34-FITC, including C34-FITC alone (line 2), N36/C34-FITC complex (line 3), and N36/C34-FITC/P20 complex (line 5). (b) The same gel then was stained with Coomassie blue to show all of the protein bands.
