FIG. 1.

RSV-induced changes of nuclear proteins of A549 cells by 2-D DIGE. Dual channel overlay of representative gel images in the pH ranges of 3.0 to 5.4 (A), 5.4 to 7.0 (B), and 7.0 to 10.0 (C). Uninfected and RSV-infected nuclear extracts were labeled with Cy3 (green) and Cy5 (red), respectively. For clarity, only a few representative spots are illustrated and found in Table 1. (D) Graphical representation of a pathway containing the largest number of focus proteins. Each node represents individual proteins, and relationships are represented by lines. Nodes colored red indicate a protein differentially upregulated by RSV, while nodes colored green indicate a downregulated protein. Uncolored nodes are inferred. Squares indicate cytokines, circles indicate chemokines, and ovals indicate transcription factors. Lines terminating with an arrow indicate “acts on.” Act6A, actin binding protein; CFL, cofilin; ECH, enoyl coenzyme A hydratase; KRT, keratin; Jnk, Jun N-terminal kinase; Prdx, peroxiredoxin; VMT, vimentin.