FIG. 3.

siRNA-mediated knockdown of Prdx-1 and Prdx-4 in RSV-treated A549 cells. Cells were transfected with 100 nM scrambled (Con), Prdx-1, Prdx-4, or Prdx-1 plus Prdx-4 siRNA. (A) After 72 h, cells were harvested and Prdx-1 and Prdx-4 were detected in whole-cell extract (WCE) by Western immunoblotting. Τhe same membrane was probed with β-actin Ab as a loading control. (B) A549 cells were transfected with either scrambled, Prdx-1, Prdx-4, or Prdx-1 plus Prdx-4 siRNA for 72 h, followed by no infection or RSV infection for 15 h. Total RNA was extracted and subjected to quantitative reverse transcription-PCR (qRT-PCR) for IL-6, Gro-β, and IL-8. Data are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and expressed as fold changes relative to the uninfected control. Each bar is the mean ± standard deviation from triplicate determinations. **, P < 0.001.