FIG. 4.

ROS production in A549 cells lacking Prdx-1, Prdx-4, or both. (A) ROS generation was measured by monitoring changes in oxidized DCF fluorescence in A549 cells transfected with control, Prdx-1, Prdx-4, or both Prdx-1 and Prdx-4 siRNA, after which the cells were infected with RSV for 15 h. Bars represent the means ± standard errors of two independent measurements in duplicate. H₂O₂ was used as a positive control. (B) Enhanced carbonylation of proteins by RSV infection in Prdx-1, Prdx-4, or Prdx-1 plus Prdx-4 knockdown cells by Western blotting. Cellular extracts from an experiment identical to that described for panel A were subjected to SDS-PAGE and transferred to a PVDF membrane. The membrane was probed with anti-DNP antibody. GAPDH is shown as a loading control.