TABLE 2.

Identification of proteins whose cysteine residues were protected from oxidation by either Prdx-1, Prdx-4, or both in RSV-infected A549 cells^a

Protein name	Common name	Accession no.	Expectation score	Protection index
				Control	Prdx-1 siRNA	Prdx-4 siRNA	Prdx-1 + Prdx-4 siRNA	Prdx-1, Prdx-1 + Prdx-4 or Prdx 4, Prdx-1 + Prdx-4 <−1.25
Carbamoyl-phosphate synthase	CPSM	P31327	3.20E−41	1.29	−1.16	−1.50	−3.07	−3.07
Leucine-rich PPR motif-containing protein	LPPRC	P42704	8.00E−84	−1.08	−2.59	−1.29	−1.27	−2.59
Stress-70 protein	GRP75	P38646	5.00E−12	−1.26	1.37	−1.69	−1.39	−1.69
Desmoplakin	DESP	P15924	1.00E−19	−2.87	1.13	−1.87	−1.84	−1.87
70-kDa lamin	LMNA	P02545	6.30E−16	1.50	1.13	−2.70	−1.64	−2.70
ATP synthase subunit alpha	ATPA	P25705	8.00E−43	−1.29	1.05	−2.97	−1.43	−2.97
Sulfide:quinone oxidoreductase	SQRD	Q9Y6N5	6.30E−35	1.34	−1.01	−1.58	−2.73	−2.73
Aspartate aminotransferase	AATM	P04259	1.60E−17	1.33	−1.37	1.76	−1.72	−1.72
Annexin A2	ANXA2	P07355	2.00E−22	18.90	−1.72	1.98	−1.33	−1.72
Centromere-associated protein E	CENPE	Q02224	8.00E−07	2.06	1.90	−1.86	−1.35	−1.86
Periplakin	PEPL	O60437	3.80E−06	−1.05	1.11	−3.92	−1.50	−3.92
Centromere-associated protein E	CENPE	Q02224	8.00E−09	4.36	−1.75	2.00	−1.25	−1.75
Centromere-associated protein E	CENPE	Q02224	3.90E−05	1.35	1.28	−2.29	−1.36	−2.29
Enoyl-CoA hydratase	ECHA	P40939	8.00E−48	1.60	−1.34	−1.03	−2.20	−2.20
Desmoplakin	DESP	P15924	3.20E−64	−2.23	1.44	−3.52	−2.17	−3.52
Glial fibrillary acidic protein	GFAP	P14136	1.30E−08	−1.78	−2.14	1.23	−1.32	−2.14
Glial fibrillary acidic protein	GFAP	P14136	3.60E−05	−1.32	−1.23	−1.29	−5.57	−5.57
Annexin A2	ANXA2	P04259	3.20E−18	−8.91	−2.61	−4.61	−1.61	−4.61
Centrosomal protein (290 kDa)	CE290	O15078	2.10E−05	−1.27	−1.40	−3.60	−1.51	−3.60
Peroxiredoxin-5	Prdx5	P30044	5.00E−14	−2.48	−1.05	−1.35	−10.95	−10.95

^aProtection was determined by the ratio of spot volume of a protein labeled with BODIPY-FL-maleimide to Sypro ruby. A protection index is presented in the far right section of the table by number (a positive value indicates protection, while a negative value indicates Cys oxidation).